Transfection Reagents
EDITCO BIO INC Transfection Optimization Kit (Multi-guide)
The Transfection Optimization Kit is a validated positive control kit based on Synthego’s multi-guide strategy that targets human TRAC gene. Recommend using this kit to optimize transfection conditions for your cell type prior to using the Gene Knockout Kit v2. Consider a cell line has been optimized when you achieve at least 50% editing efficiency for TRAC positive control.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT DUO TRANSRGNT .75ML
DharmaFECT Duo Transfection Reagent 0.75mL
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit R
The Cell Line Nucleofector Kit R is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:B16-F0BJCOS-7HeLaHT-29Hut-78IMR-90KG-1L6LNCaPMDBKNIH/3T3NK-92PANC-1Sf9WI-38 The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution R0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Lonza Walkersville Mouse Embryonic Fibroblast Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast Nucleofector Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells -Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Embryonic Fibroblasts0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Qiagen, Inc. EFFECTENE TRANSFCT.REAGENT 1ML
Effectene Transfection Reagent, no complex removal needed for most cell lines, fast and easy transfection, high efficiencies with a wide variety of cell types, contains: 1mL effective Reagent, Enhancer, Buffer; for 40 transfections in 60mm
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.
LAMDA BIOTECH INC Tranfection reagent: SusFexin
SusFexin is a biodegradable polymer based transfection reagent for suspension cell transfection. When mix with DNA, it will form complex with DNA and transport the complex into a variety of suspension cell lines. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection, leading to a much less cytotoxicity to the transfected cells and improving transfection efficiency and productivity of trans-gene expression.
Takara Bio Xfect™ RNA Transfection Reagent | 1.2mL
Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA, including messenger RNA (mRNA), small interfering RNA (siRNA), and single guide RNA (sgRNA).The protocol is simple, and transfections can be carried out entirely in the presence of serum. Xfect RNA Transfection Reagent creates biodegradable nanoparticles when combined with RNA, resulting in very low cytotoxicity.
HORIZON DISCOVERY BIOSCIENCES LIMITED Accell Green Non-targeting Control siRNA, 20nmol
Accell Green Non-targeting Control siRNA provides qualitative assessment of Accell siRNA uptake as determined by fluorescent microscopy to evaluate cytoplamic localization of the dye-labeled Accell siRNA or FACS analysis to determine general uptake. Non-targeting siRNA reagents are designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses.
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products